Chemical Stability and Antimicrobial Activity of Plasma-Sprayed Cerium Oxide–Incorporated Calcium Silicate Coating in Dental Implants. Issue 6 (December 2019)

• Record Type: Journal Article
• Authors: Qi, Shengcai; Wu, Jinjin; Xu, Yiwen; Zhang, Yiming; Wang, Raorao; Li, Kai; Xu, Yuanzhi
• Is Part Of: Implant dentistry. Volume 28:Issue 6(2019)
• Publication Date: 2019-12
• Subjects: CeO2 -- antibacterial activity -- Enterococcus faecalis; Dental implants -- Periodicals; 617.69305
• DOI: 10.1097/ID.0000000000000937
• Languages: English
• ISSNs: 1056-6163

Abstract

Abstract : Purpose: The aim of this study is to investigate the biological activity and antibacterial property of cerium oxide–incorporated calcium silicate coatings (CeO2-CS) in dental implants. Materials and Methods: In this study, MC3T3-E1 cells cultured on the plastic, Ti-6Al-4V, and the cerium oxide–incorporated calcium silicate coatings (CeO2-CS) coating served as the blank, control, and CeO2-CS groups, respectively. A cell counting kit-8 (CCK-8) and flow cytometry were used to evaluate the biocompatibility. The osteoblastic differentiation of the MC3T3-E1 cells was also analyzed by quantitative real-time polymerase chain reaction analysis. The CCK-8 and counts of colony-forming units (CFUs) were used to detect the antibacterial activity of the coating on Enterococcus faecalis . The study showed that the cerium oxide–incorporated calcium silicate coating (CeO2-CS) has better biocompatibility. Meanwhile, the ALP, OCN, and BSP mRNA expression levels in the CeO2-CS group were significantly upregulated ( P < 0.05). The number of viable bacteria and the CFU results were significantly reduced in the CeO2-CS group ( P < 0.05). Conclusion: The cerium oxide–incorporated calcium silicate coatings (CeO2-CS) may promote the osteoblastic differentiation of osteoblasts. Meanwhile, the cerium oxide–incorporated calcium silicate coating (CeO2-CS) showed strong antimicrobial activity on E. faecalis, with good biocompatibility.