Contrasting anther glucose‐6‐phosphate dehydrogenase activities between two bean varieties suggest an important role in reproductive heat tolerance. (2nd May 2021)

• Record Type: Journal Article
• Authors: Santiago, James P.; Soltani, Ali; Bresson, Madeline M.; Preiser, Alyssa L.; Lowry, David B.; Sharkey, Thomas D.
• Is Part Of: Plant, cell and environment. Volume 44:Number 7(2021)
• Page Start: 2185
• Page End: 2199
• Publication Date: 2021-05-02
• Subjects: anther metabolism -- antioxidant enzymes -- heat stress -- pollen development -- reactive oxygen species -- ROS quenching -- sucrose phloem loading; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.14057
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Common beans ( Phaseolus vulgaris ) are highly sensitive to elevated temperatures, and rising global temperatures threaten bean production. Plants at the reproductive stage are especially susceptible to heat stress due to damage to male (anthers) and female (ovary) reproductive tissues, with anthers being more sensitive to heat. Heat damage promotes early tapetal cell degradation, and in beans this was shown to cause male infertility. In this study, we focus on understanding how changes in leaf carbon export in response to elevated temperature stress contribute to heat‐induced infertility. We hypothesize that anther glucose‐6‐phosphate dehydrogenase (G6PDH) activity plays an important role at elevated temperature and promotes thermotolerance. To test this hypothesis, we compared heat‐tolerant and susceptible common bean genotypes using a combination of phenotypic, biochemical, and physiological approaches. Our results identified changes in leaf sucrose export, anther sugar accumulation and G6PDH activity and anther H2 O2 levels and antioxidant‐related enzymes between genotypes at elevated temperature. Further, anther respiration rate was found to be lower at high temperature in both bean varieties. Overall, our results support the hypothesis that enhanced male reproductive heat tolerance involves changes in the anther oxidative pentose phosphate pathway, which supplies reductants to critical H2 O2 scavenging enzymes. Abstract : In this article, we describe the contribution of G6PDH in maintenance of ROS levels and by extension, its role in viable pollen production as a measure of heat tolerance in common beans. Our data indicate that continued flux of carbon through G6PDH in anthers helps remove hydrogen peroxide at high temperature in heat‐tolerant bean genotype.