Characterization of bio‐fabricated silver nanoparticles for distinct anti‐fungal activity against sugarcane phytopathogens. Issue 7 (20th February 2021)

• Record Type: Journal Article
• Authors: Amna, ; Mahmood, Tariq; Khan, Umar Nawaz; Amin, Babar; Javed, Muhammad Tariq; Mehmood, Shehzad; Farooq, Muhammad Asad; Sultan, Tariq; Munis, Muhammad Farooq Hussain; Chaudhary, Hassan Javed
• Is Part Of: Microscopy research and technique. Volume 84:Issue 7(2021)
• Page Start: 1522
• Page End: 1530
• Publication Date: 2021-02-20
• Subjects: antifungal -- Colletotricum falcatum -- Fusarium moniliforme -- pathogens -- silver nanoparticles; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23708
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Advanced research, development, and application of silver nanoparticles is proceeding in recent times due to their incredible utilization in various fields. Present study was focused on the production, characterization, and antifungal activities of silver nanoparticles (AgNPs). An environment friendly extracellular biosynthetic approach was adopted to produce the AgNPs by using bacteria, fungi, and sugarcane husk. Agents used for reduction of silver to nanoparticles were taken from culture filtrate of plant growth promoting bacteria, Fusarium oxysporum and supernatant extract of sugarcane husk. Nanoparticles were also characterized by scanning electron microscopy (SEM). Synthesis of colloidal AgNPs was observed by UV–Visible diffused reflectance spectroscopy (UV–Vis DRS). Primary peak of surface plasmon resonance band was noticed around 339.782, 336.735, and 338.258 nm for bacterial, fungal, and sugarcane husk produced AgNPs. Structure of all biologically produced nanoparticles were crystalline cubic with nano size of 45.41, 49.06, and 42.75 nm for bacterial, fungal, and sugarcane husk‐based nanoparticles, respectively as calculated by Debye–Scherrer equation using XRD. Fourier transform infrared spectroscopy (FTIR) analysis revealed the presence of various compounds that aid in the reduction, capping, and stability of AgNPs. The antifungal activity of AgNPs was also investigated for sugarcane fungal pathogens Colletotricum falcatum and Fusarium moniliforme . All nanoparticles exhibit prominent antifungal activities. Maximum zone of fungal inhibition was noticed about 18, 19, and 21 mm for C. falcatum while 21, 20, and 24 mm for F. moniliforme in case of bacterial, fungal, and plant‐based nanoparticles (15 ppm), respectively. Best fungal inhibition was observed under application of sugarcane husk based AgNPs. Moreover, biologically produced AgNPs responded better towards the suppression of F. moniliforme in comparison to C. falcatum . Mentioned sources in present study can be ecofriendly nano‐factories for biosynthesis of AgNPs and mankind should benefit from their commercial application. Abstract : Biosynthesis and characterization of silver nanoparticles. Biofabricated silver nanoparticles showed distinct inhibition of Colletotricum falcatum and Fusarium moniliforme . Pathogen inhibition is dominant in sugarcane husk based nanoparticles.