Cytotoxic effects of Satureja montana L. essential oil on oocytes of engorged Rhipicephalus microplus female ticks (Acari: Ixodidae). Issue 7 (6th January 2021)

• Record Type: Journal Article
• Authors: Reis, Aline Chaves; Konig, Isaac Filipe Moreira; Rezende, Danúbia Aparecida de Carvalho Selvati; Gonçalves, Raquel Romano Palmeira; Lunguinho, Allan da Silva; Ribeiro, Jenifer Caroline Silva; Cardoso, Maria das Graças; Remedio, Rafael Neodini
• Is Part Of: Microscopy research and technique. Volume 84:Issue 7(2021)
• Page Start: 1375
• Page End: 1388
• Publication Date: 2021-01-06
• Subjects: cattle tick -- essential oil -- ovary morphology -- Satureja montana L. -- tick control; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23693
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: In addition to pesticidal activity, the capacity of natural compounds to inhibit the reproduction of parasites emerge as an important alternative tick control method. In this context, Satureja spp. stand out due to their recognized pesticidal properties. Among parasites of veterinary importance, the cattle tick, Rhipicephalus microplus, is responsible for great economic losses in livestock and transmission of relevant pathogens. Thus, the aim of this study was to evaluate the effects of different concentrations of the essential oil of Satureja montana L. on the ovary morphology of R. microplus engorged females through histological and histochemical techniques. The most remarkable morphological changes found were: cytoplasmic vacuolation of germ cells, irregular and thicker chorion, irregular oocyte shape, ring‐shaped nucleolus, decrease in protein and carbohydrate content in oocytes, in addition to cellular changes in the oviduct and pedicel. All morphological changes were assessed using a semiquantitative method already established in the literature. Ticks exposed to 5.0 μl/ml of essential oil showed the most significant changes when compared to control groups. Thus, the essential oil of S. montana L. damaged the reproductive system of R. microplus, which may impair ticks' offspring production and promote a long‐term control of this species. Highlights: The essential oil of Satureja montana L. affects the ovary morphology of the cattle tick. The main morphological alterations found were cytoplasmic vacuolation, irregular and thicker chorion and irregular oocyte shape. These alterations may impair the development of eggs. Abstract : Effects of Satureja montana L. essential oil on cattle tick reproduction.