Corynebacterium endometrii sp. nov., isolated from the uterus of a cow with endometritis. Issue 1 (4th October 2019)

• Record Type: Journal Article
• Authors: Ballas, Panagiotis; Rückert, Christian; Wagener, Karen; Drillich, Marc; Kämpfer, Peter; Busse, Hans-Jürgen; Ehling-Schulz, Monika
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 146
• Page End: 152
• Publication Date: 2019-10-04
• Subjects: Corynebacterium -- endometritis -- bovine; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003728
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive bacterial strain, designated LMM-1653 T, was isolated from a uterus swab from a Holstein Frisian dairy cow in the frame of a clinical sampling trial. The isolated strain, which showed a rod to coccoid shape, was catalase-positive and oxidase-negative. Based on 16S rRNA gene sequence similarity, its closest relatives were Corynebacterium flavescens and Corynebacterium argatoratense (96.50 % similarity each), suggesting that this isolate represents a novel species. Strain LMM-1653 T had a quinone system consisting mainly of menaquinones MK-8(H2 ) and MK-9(H2 ). The polar lipid profile showed presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside as well as one unidentified glycolipid and one unidentified aminoglycolipid. Moderate to minor amounts of three unidentified glycolipids, β-gentiobiosyl diacylglycerol, one unidentified aminoglycolipid and three unidentified lipids without a functional group were also found. The cell wall contained meso-diaminopimelic acid and the strain also contained corynemycolic acids. The fatty acid profile was predominantly composed of straight-chain, saturated and mono-unsaturated fatty acids, dominated by C18 : 1 ω9 c and C16 : 0 . Since this isolate differs from the nearest related established Corynebacterium species in its genetic and phenotypic traits, a novel species named Corynebacterium endometrii LMM-1653 T (=LMG-31164 T =CCM 8952 T ) of the genus Corynebacterium is proposed.