Emendation of the Genus Auritidibacter Yassin et al. 2011 and Auritidibacter ignavus Yassin et al. 2011 based on features observed from Canadian and Swiss clinical isolates and whole-genome sequencing analysis. Issue 1 (8th October 2019)

• Record Type: Journal Article
• Authors: Bernard, K. A.; Pacheco, A. L.; Burdz, T.; Wiebe, D.; Beniac, D. R.; Hiebert, S. L.; Booth, T. F.; Jakopp, B.; Goldenberger, D.; Seth-Smith, H. M. B.; Egli, A.; Bernier, A-M
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 83
• Page End: 88
• Publication Date: 2019-10-08
• Subjects: Auritidibacter ignavus -- otitis media -- Genus Auritidibacter; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003719
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Auritidibacter ignavus is a Gram-stain-positive bacillus derived from otorrhea. Four strains derived from ear discharges in Canada and Switzerland, with features consistent with but distinguishable from Auritidibacter ignavus IMMIB L-1656 T (accession number FN554542) by 16S rRNA gene sequencing (97.5 % similarity), were thought to represent a novel species of the genus Auritidibacter . Auritidibacter ignavus DSM 45359 T (=IMMIB L-1656 T ) was acquired to compare with Canadian and Swiss strains by whole-genome sequencing (WGS). Unexpectedly, those isolates were observed to be consistent with A. ignavus DSM 45359 T by WGS (ANIb scores >98 %), MALDI-TOF (Bruker), cellular fatty acid analysis and biochemically (some differences were observed). A nearly full 16S rRNA gene sequence could not be readily prepared from A. ignavus DSM 45359 T, even after multiple attempts. A 16S rRNA gene chimeric consensus sequence created from the genome assembly of A. ignavus DSM 45359 T had only 97.5 % similarity to that of A. ignavus IMMIB L-1656 T, implying that 16S rRNA sequence accession number FN554542 could not be replicated. We concluded that our isolates of members of the genus Auritidibacter were consistent with A. ignavus DSM 45359 T, did not represent a novel species, and that the sequence corresponding to FN554542 was not reproducible. By WGS, A. ignavus DSM 45359 T had genome of 2.53×10 6 bp with a DNA G+C content of 59.34%, while genomes of Canadian and Swiss isolates ranged from 2.47 to 2.59×10 6 bp with DNA G+C contents of 59.3–59.52 %. A. ignavus NML 100628 (=NCTC 14178=LMG 30897) did not demonstrate a rodcoccus cycle. Emendation of Auritidibacter ignavus was proposed based on these results.