Marinobacter fonticola sp. nov., isolated from deep sea cold seep sediment. Issue 2 (28th November 2019)

• Record Type: Journal Article
• Authors: Sun, Qing-lei; Sun, Yan-ling; Sun, Yuan-yuan; Luan, Zhen-dong; Lian, Chao
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 2(2020)
• Page Start: 1172
• Page End: 1177
• Publication Date: 2019-11-28
• Subjects: cold seep -- Marinobacter -- deep sea; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003895
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : In this study, we report a novel Gram-negative bacterium, designated as strain CS412 T, isolated from deep-sea sediment collected in a cold seep area of the South China Sea. Growth of strain CS412 T occurred at 4–40 °C (optimum, 28 °C), pH 5.0–11.0 (optimum, pH 6.0) and with 0–19 % (w/v) NaCl (optimum, 1–2 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain CS412 T belonged to the genus Marinobacter . The closest phylogenetic neighbours of strain CS412 T were Marinobacter pelagius HS225 T (96.9 %), Marinobacter szutsaonensis NTU-104 T (96.8%), Marinobacter santoriniensis NKSG1 T (96.4%) and Marinobacter koreensis dd -M3 T (96.3 %). The genomic DNA G+C content of strain CS412 T was 58.0 mol%. The principal respiratory quinone was ubiquinone-9 (Q-9). The polar lipids of CS412 T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipidand and four glycolipids. The major fatty acids of CS412 T contained cyclo-C19 : 0 ω8 c, C16 : 0, C18 : 1 ω7 c and C18 : 1 ω7 c 11-methyl. The results of phylogenetic, physiological, biochemical and morphological analyses suggested that strain CS412 T represents a novel species of the genus Marinobacter, and the name Marinobacter fonticola sp. nov. is proposed with the type species CS412 T (=CCTCC AB 2019197 T =KCTC 72475 T ).