Serinibacter arcticus sp. nov., isolated from a thawing ancient ice wedge. Issue 2 (15th November 2019)

• Record Type: Journal Article
• Authors: Filippova, Svetlana N.; Surgucheva, Natalya A.; Detkova, Ekaterina N.; Rakitin, Andrey L.; Beletsky, Aleksey V.; Grouzdev, Denis S.; Kolganova, Tatyana V.; Mulyukin, Andrey L.
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 2(2020)
• Page Start: 929
• Page End: 934
• Publication Date: 2019-11-15
• Subjects: actinobacteria -- Serinibacter arcticus sp. nov. -- ice wedge -- Mammoth Mountain; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003848
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel actinobacterium, strain K3-2 T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia). Colonies of strain K3-2 T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming. Strain K3-2 T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С. The cell-wall peptidoglycan of strain K3-2 T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine. The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids. The major fatty acids were anteiso-C15 : 0 and C16 : 0 . The only menaquinone detected was MK-8(H4 ). 16S rRNA gene analysis indicated that strain K3-2 T belongs to the genus Serinibacter . The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28 T and Serinibacter tropicus PS-14-7 T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively. The average nucleotide identity value of the whole genome sequence between strain K3-2 T and S. salmoneus Kis4-28 T was 78.9 %. DNA–DNA relatedness values between strain K3-2 T and S. salmoneus DSM 21801 T (=Kis4-28 T ) and S. tropicus VKPM Ac 2044 T (=PS-14-7 T ) were 41 and 47 %. Thus, strain K3-2 T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp. nov. is proposed. The type strain is K3-2 T (DSM 103859 T =VKM Ас−2719 T ).