Dyadobacter flavalbus sp. nov., isolated from lake sediment. Issue 2 (21st November 2019)

• Record Type: Journal Article
• Authors: Qu, Jian-Hang; Yue, Yi-Fei; Zhou, Jia; Qu, Ling-Bo; Wang, Lin-Feng
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 2(2020)
• Page Start: 1064
• Page End: 1070
• Publication Date: 2019-11-21
• Subjects: Dyadobacter flavalbus -- lake sediment -- polyphasic identification; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003876
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel bacterial strain, designated NS28 T, was isolated from interfacial sediment sampled at Taihu Lake, PR China. Cells were rod-shaped, Gram-negative, aerobic and non-motile on Reasoner's 2A medium. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NS28 T was most closely related to species from the genus Dyadobacter, with 98.4 and 96.0 % 16S rRNA gene sequence similarity to its closest phylogenetic neighbours Dyadobacter sediminis CGMCC 1.12895 T and Dyadobacter luticola CCTCC AB 2017091 T, respectively. MK-7 was the only cellular menaquinone. The major fatty acids were summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6 c ), iso-C15 : 0 and C16 : 1 ω5 c . The major polar lipids were phosphatidylethanolamine, one phospholipid, one aminolipid, one lipid and two unidentified lipids. Genomic analysis of strain NS28 T indicated that the total genome size was 6 477 094 bp with a G+C content of 44.8 mol%, 5380 protein-coding genes, 79 contigs and an N50 length of 299584 bp. On the basis of the genomic DNA sequence, the average nucleotide identity values were 90.5 and 74.1 % with D. sediminis CGMCC 1.12895 T and D. luticola CCTCC AB 2017091 T, respectively. Digital DNA-DNA hybridization results of strain NS28 T with D. sediminis CGMCC 1.12895 T and D. luticola CCTCC AB 2017091 T were 40.9 and 18.6 %, respectively. Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence data presented here, it is proposed that strain NS28 T represents a novel species of the genus Dyadobacter for which the name Dyadobacter flavalbus is proposed . The type strain is NS28 T (=NBRC 113854 T =MCCC 1K03764 T ).