One-pot surface modification of magnetic nanoparticles using phase-transitioned lysozyme for robust immobilization of enzymes. (8th June 2021)

• Record Type: Journal Article
• Authors: Li, Ling; Gao, Zixi; Zhang, Huige; Du, Hongying; Ren, Cuiling; Qi, Shengda; Chen, Hongli
• Is Part Of: New journal of chemistry. Volume 45:Number 25(2021)
• Page Start: 11153
• Page End: 11159
• Publication Date: 2021-06-08
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d1nj00957e
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Enzymes were one-pot immobilized between Fe3 O4 nanoparticles and a phase-transitioned lysozyme film, providing a new strategy for enzyme immobilization. Abstract : As free enzymes are often limited due to the poor operational stability, high cost and no reusability, immobilization of enzymes is a preferred way to overcome the obstacles. Herein, a 2D protein self-assembly film was first reported to capture functional enzymes without any further chemical modification. Enzymes are immobilized between Fe3 O4 nanoparticles and the robust phase-transitioned lysozyme film, which prevented the leaching of enzymes and ensured the contact of enzymes and substrates. The immobilized enzyme was characterized by FT-IR, TEM, TGA and VSM analysis. Furthermore, the pH stability, thermal stability, storage stability and reusability were greatly improved after the immobilization of the enzyme. Our work provides a nontoxic and inexpensive strategy for enzyme immobilization, which has promising potential in inhibitor screening and enzyme engineering.