Two-dimensional ultrafast transient absorption spectrograph covering deep-ultraviolet to visible spectral region optimized for biomolecules. (21st June 2021)

• Record Type: Journal Article
• Authors: Nazari Haghighi Pashaki, Maryam; Mosimann-Schönbächler, Nina; Riede, Aaron; Gazzetto, Michela; Rondi, Ariana; Cannizzo, Andrea
• Is Part Of: JPhys photonics. Volume 3:Number 3(2021)
• Publication Date: 2021-06-21
• Subjects: UV two-dimensional spectroscopy -- ultrafast transient absorption spectroscopy -- deep-UV time resolved spectroscopy -- phenanthrene multichromophoric system -- azurin; Photonics -- Periodicals; 621.365
• DOI: 10.1088/2515-7647/ac0805
• Languages: English
• ISSNs: 2515-7647

Abstract

Abstract: We report on the implementation of a multi-kHz single-shot referenced non-coherent two-dimensional UV spectrograph based on conventional pump-probe geometry. It has the capability to cover a broad spectral region in excitation from 270-to-380 nm and in the detection from 270-to-390 nm and 320-to-720 nm. Other setups features are: an unprecedented time resolution of 33 fs (standard deviation); signals are photometrically corrected; a single-shot noise of <1 mOD. It has the capability to operate with sample volumes as small as few μ l which is an accomplishment in studying biological or biomimetic systems. To show its performances and potentials, we report two preliminary studies on the photophysics of phenanthrenes hosted in a multichromophoric antenna system and of aromatic amino acids in a blue-copper azurin.