In vitro and in vivo degradability, biocompatibility and antimicrobial characteristics of Cu added iron-manganese alloy. (10th September 2021)

• Record Type: Journal Article
• Authors: Mandal, Santanu; Kishore, Vijay; Bose, Madhuparna; Nandi, Samit Kumar; Roy, Mangal
• Is Part Of: Journal of materials science & technology. Volume 84(2021)
• Page Start: 159
• Page End: 172
• Publication Date: 2021-09-10
• Subjects: Iron-manganese alloy -- Degradable metal -- In vitro -- Micro-CT; Metals -- Periodicals; Materials science -- Periodicals; Materials science; Metals; Periodicals; 620.1105
• DOI: 10.1016/j.jmst.2020.12.029
• Languages: English
• ISSNs: 1005-0302

Abstract

Graphical abstract: Highlights: Slow degradation is one of the primary concerns of Fe-Mn system. Fe-Mn-Cu samples were processed through melting-casting-forging route to achieve better control over its properties. Corrosion rate of cast Fe-Mn-10Cu alloy is increased (0.072 mmpy) compared to base alloy (0.058 mmpy). Addition of Cu significantly reduced bacterial growth. In vivo degradation and biocompatibility were confirmed by implanting in rabbit femur. Abstract: In recent years, iron (Fe) based degradable metal is explored as an alternative to permanent fracture fixation devices. In the present work, copper (Cu) is added in Fe-Mn system to enhance the degradation rate and antimicrobial properties. Fe-Mn- x Cu ( x = 0.9, 5 and 10 wt.%) alloys are prepared by the melting-casting-forging route. XRD analysis confirms austenite phase stabilization due to the presence of Mn and Cu. As predicted by Thermo-Calc calculations, Cu rich phase precipitations are noticed along the austenite grain boundaries. Degradation behaviours of Cu added Fe-Mn alloys are investigated through static immersion and electrochemical polarization where enhanced degradation is found for higher Cu added alloys. When challenged against E.Coli bacteria, the Fe-Mn-Cu alloy media extract shows a significant bactericidal effect compare to the base alloy. In vitro cytocompatibility studies, as determined using MG63 and MC3T3-E1 cell lines, indicate increased cell density as a function of time for all the alloys. When implanted in rabbit femur, the newly developed alloy does not show any kind of tissue necrosis around the implants. Better osteogenesis and higher new bone formation are observed with Fe-Mn-10Cu alloy as evident from micro-computed tomography (μ-CT) and fluorochrome labelling.