Selective, stoichiometric and fast-response fluorescent probe based on 7-nitrobenz-2-oxa-1, 3-diazole fluorophore for hypochlorous acid detection. (September 2021)

• Record Type: Journal Article
• Authors: Świerczyńska, Małgorzata; Słowiński, Daniel; Grzelakowska, Aleksandra; Szala, Marcin; Romański, Jarosław; Pierzchała, Karolina; Siarkiewicz, Przemysław; Michalski, Radosław; Podsiadły, Radosław
• Is Part Of: Dyes and pigments. Volume 193(2021)
• Publication Date: 2021-09
• Subjects: Fluorescent probe -- Thiomorpholine-based probes -- 7-Nitrobenz-2-oxa-1, 3-diazole -- Hypochlorous acid -- Stoichiometric reaction -- Myeloperoxidase; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.109563
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A new selective probe containing the thiomorpholine unit (TM) was synthesized and examined for the fluorescence-based detection of hypochlorous acid (HOCl). Connection of TM with the fluorophore, 7-nitrobenz-2-oxa-1, 3-diazole (NBD) effectively suppresses NBD fluorescence, whereas a HOCl-induced oxidation of TM to its S -oxide turns on a green emission. HPLC analysis shows that the reaction is stoichiometric and no sulfone is formed. Moreover, neither hydrogen peroxide nor peroxynitrite are able to oxidize the probe. Experimental data indicates that NBD-TM probe can be used to detect HOCl generated by myeloperoxidase (MPO). Presented results may be helpful in developing a novel class of fluorescent and luminogenic probes for the imaging of HOCl. Graphical abstract: Image 1 Highlights: NBD-derived probe for detection of hypochlorous acid. Thiomorpholine unit (TM) was used as HOCl-selective group. TM effectively quenches the fluorescence of NBD. HOCl oxidazes NBD-TM stoichiometrically to its sulfoxide and turns on emission. NBD-TM probe enables real-time monitoring of the HOCl generated enzymatically by myeloperoxidase.