Robust Biocatalysts Displayed on Crystalline Protein‐Layered Cells for Efficient and Sustainable Hydration of Carbon Dioxide. (15th April 2021)

• Record Type: Journal Article
• Authors: Koo, Bon Il; Choi, Jae Woong; Song, Seuk Young; Choi, Ye Hun; Lee, Tae Yong; Kim, Shin‐Hyun; Jeong, Ki Jun; Nam, Yoon Sung
• Is Part Of: Advanced functional materials. Volume 31:Number 25(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-04-15
• Subjects: carbon dioxide capture -- carbonic anhydrase -- cell display -- Corynebacterium -- enzyme thermostability; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202102497
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Mineral carbonation is the most effective carbon capture technique, but carbon dioxide (CO2 ) conversion is limited by the slow hydration rate of CO2 (<10 −1 s −1 ). A biological solution exists: carbonic anhydrase (CA) efficiently hydrates CO2 at a turnover rate of ≈10 6 s −1 under ambient conditions, making it an extremely attractive candidate for industrial post‐combustion CO2 capture. However, high cost and poor long‐term stability impose a technical barrier to its practical uses. Here, a genetically engineered Corynebacterium glutamicum ( C. glutamicum ) is introduced as a robust cell display platform for the in situ stabilization and low‐cost production of CA. The enzyme is displayed in the mycolic layer with porin B as an anchoring protein with (GGGGS)2 as a spacer. The cell‐displayed CA exhibits no significant inactivation of the CO2 hydration activity for at least one month at 37 °C. Its denaturation rate constant at 50 °C (0.07) is an order of magnitude lower than that of free CA (0.52–0.54). This study demonstrates that a structurally robust cell template allows the effective stabilization of CA, suggesting the C. glutamicum ‐based cell display as a promising technique to achieve highly efficient, sustainable, and low‐cost CO2 capture for industrial applications. Abstract : Robust biocatalysts displayed on crystalline protein‐layered cells, which is the first successful attempt to harness genetically engineered Corynebacterium glutamicum as a structurally robust cell‐display platform, are devised for the in situ stabilization of carbonic anhydrase, low‐cost production, and efficient, sustainable hydration of carbon dioxide.