DNA‐Based Architectures for in situ Target Biomolecule Analysis in Confined Nano‐space†. Issue 7 (18th June 2021)

• Record Type: Journal Article
• Authors: Hu, Xiaoxue; Huang, Yide; Yin, Hao; Dai, Lizhi; Tian, Ye
• Is Part Of: Chinese journal of chemistry. Volume 39:Issue 7(2021)
• Page Start: 2027
• Page End: 2034
• Publication Date: 2021-06-18
• Subjects: Nanotechnology -- Biomolecule analysis -- DNA structures -- Fluorescent probes -- Confined nanospace; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cjoc.202000753
• Languages: English
• ISSNs: 1001-604X

Abstract

Abstract: In situ target biomolecule analysis is of great significance for real‐time monitoring and regulation of endogenous biomarkers and elementary biomolecules in vivo . Gratifyingly, the rapid evolution of structural DNA nanotechnology during past decades has established an appealing toolbox for biological analysis and medical detection. The modulated self‐assembly and underlying canonical Watson‐Crick base‐pairing rules provide possibilities for accurate controlling of the topologies and functions of obtained nanomaterials. The probes composed of diverse DNA nanostructures and DNA‐nanoparticle complexes can create a confined space, which increases target accessibility and improves probe stability, sensitivity and specificity. In this minireview, we retrospect the research progress of in‐situ biomolecular analysis based on DNA nanostructures for intracellular and in vivo biosensors in confined space. The characteristics of distinct DNA nanomaterials are first introduced, and then the fundamentals of biosensing process of designed DNA nanostructures are emphasized. Moreover, we elucidate our perspective over the challenges of this field and discuss the potential directions of this kind of application‐oriented fabrication technique. Abstract : Structural DNA nanotechnology has established an attractive toolbox for biological analysis and medical detection. In this mini review, we focus on the research progress of in situ analysis of DNA nanostructured biosensors in confined space.