Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. (15th September 2021)

• Record Type: Journal Article
• Authors: Wang, Chengxiao; Liu, Shengnan; Xu, Junwei; Gao, Mingju; Qu, Yuan; Liu, Yuan; Yang, Ye; Cui, Xiuming
• Is Part Of: Carbohydrate polymers. Volume 268(2021)
• Publication Date: 2021-09-15
• Subjects: Panax notoginseng polysaccharide -- Microneedles -- Transdermal drug delivery -- Skin dendritic cells -- Transcutaneous immunization; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118211
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: This work explored the feasibility of using biological polysaccharide to fabricate dissolvable microneedles (MNs) for the purpose of transdermal drug delivery and skin dendritic cell (DC) activation. Panax notoginseng polysaccharide (PNPS), a naturally derived immunoactive macromolecule, was used to fabricate dissolvable MNs. The prepared PNPS MNs showed a satisfactory mechanical strength and a skin penetration depth. By Franz diffusion cell assay, the PNPS MNs demonstrated a high transdermal delivery amount of model drugs. Furthermore, with the assistance of MNs, PNPS easily penetrated across the stratum corneum and target ear skin DCs, activating the maturation and migration of immunocytes by increasing the expressions of CD40, CD80, CD86, and MHC II of skin DCs. Consequently, the matured DCs migrated to the auricular draining lymph nodes and increased the proportions of CD4+ T and CD8+ T cells. Thus, PNPS might be a promising biomaterial for transdermal drug delivery, with adjuvant potential. Highlights: Panax notoginseng polysaccharide (PNPS) is used to fabricate dissolved microneedles (MNs). The PNPS MNs showed satisfied mechanical strength and skin puncture ability. The PNPS MNs showed good transdermal drug delivery profiles. The PNPS can target and activate the skin dendritic cells.