Sub-chronic exposure to PhIP induces oxidative damage and DNA damage, and disrupts the amino acid metabolism in the colons of Wistar rats. (July 2021)

• Record Type: Journal Article
• Authors: Zhao, Xiuli; Shao, Zeping; Zhou, Xiaofei; Lin, Yongxi; Guo, Jingting; Guo, Jun; Zhang, Yan; Wang, Shuo
• Is Part Of: Food and chemical toxicology. Volume 153(2021)
• Publication Date: 2021-07
• Subjects: PhIP -- Colon damage -- Metabolome -- Transcriptome -- Amino acid metabolism; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112249
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Heterocyclic amines (HCAs) are a group of mutagenic compounds produced during thermal processing of protein-rich foods. One of the most abundant HCAs, 2-amino-1-methyl-6-phenylimidazo[4, 5-b] pyridine (PhIP) has potential carcinogenic and mutagenic effects on human organs, especially the colon. This study aimed to explore the toxic effects of PhIP on amino acid metabolism in the colon of Wistar rats using RNA-seq and LC-MS/MS. Exposure to PhIP for 4 weeks induced oxidative damage and DNA damage in the colons, and disrupted the expression of related genes involved in tryptophan metabolism, beta(β)-alanine metabolism, valine, leucine, and isoleucine degradation, and glutathione metabolic pathways. Moreover, the levels of fecal metabolites related to amino acid metabolism were affected by PhIP. Cumulatively, these results indicate that PhIP can induce colonic oxidative injury and disorders related to amino acid metabolism, thereby providing a new theoretical basis for the study of PhIP toxicity. Graphical abstract: Image 1 Highlights: PhIP induced oxidative stress and DNA damage in colon tissue. PhIP disrupted the expression of related genes involved in amino acid metabolism. PhIP affected the levels of metabolites related to amino acid metabolism.