PpEBB1 directly binds to the GCC box-like element of auxin biosynthesis related genes. (May 2021)

• Record Type: Journal Article
• Authors: Zhao, Xuehui; Wen, Binbin; Li, Chen; Liu, Li; Chen, Xiude; Li, Dongmei; Li, Ling; Fu, Xiling
• Is Part Of: Plant science. Volume 306(2021)
• Publication Date: 2021-05
• Subjects: PpEBB1 -- PpSTY1 -- PpSRS5 -- PpYUC1 -- Plant development -- Auxin biosynthesis; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2021.110874
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Phenotype of EBB1-oe Arabidopsis is similar with overexpressing AtDRNL . EBB1 directly activates STY1, SRS5 and YUC1 by binding to the GCC box-like element. PpEBB1 could interact with PpMIEL1. Abstract: EARLY BUD-BREAK 1 (EBB1) can promote bud break, and this function is likely conserved in woody plants. To get a more comprehensive understand of its function, peach ( Prunus persica var. nectarina cultivar Zhongyou 4) PpEBB1 was overexpressed in Arabidopsis; the resultant phenotypes, including curved leaves, abnormal development of floral organs and low seed set, were similar to those of DORNRÖSCHEN-LIKE ( DRNL ) overexpression, indicating that PpEBB1 was a putative ortholog of AtDRNL. PpEBB1 bound to the GCC box-like element in the STYLISH1/SHI RELATED SEQUENCE5 ( STY1/SRS5 ) promoter of peach, which has been proposed to occur in Arabidopsis as well. A GCC box-like element was also found in the YUCCA1 ( YUC1 ) promoter, and PpEBB1 could bind to this element and activate the expression of YUC1 . In addition to the elevated auxin content in the PpEBB1-oe plants as observed in our previous study, these results suggest that PpEBB1 can regulate auxin biosynthesis by directly activating related genes. Besides, we screened a zinc finger RING-finger protein, MYB30-INTERACTING E3 LIGASE 1 (PpMIEL1), showing interaction with PpEBB1, suggesting that the stability of PpEBB1 might be influenced by PpMIEL1 through ubiquitination.