Assembly and Application of a Low Budget Photoreactor. Issue 5 (26th May 2021)

• Record Type: Journal Article
• Authors: Wallner, Olov; Mamonov, Kirill; Ortis, Florian; Michel, Dana; Michel, Maurice
• Is Part Of: Chemistry methods. Volume 1:Issue 5(2020)
• Page Start: 240
• Page End: 244
• Publication Date: 2021-05-26
• Subjects: Photolabile -- photoreactor -- protection group -- SARS-Cov-2 -- spike protein -- UV; Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1002/cmtd.202100022
• Languages: English
• ISSNs: 2628-9725

Abstract

Abstract: Photochemistry is a fast growing research field and many transformations previously not accessible to chemists now have become part of an ever growing standard repertoire. The limiting factors for a photoreactor system however are the possibility to perform stirring, removal of excess heat and the irradiation with UV or visible light – all that within a secure surrounding. Systems for starters may be as expensive as several thousand Euro. Here we design and assemble a LED photoreactor using scrap and standard materials, spending less than 30 € for a LED. The system may be adjusted to any required wavelength and its assembly is shown for the use of a 400 nm LED. To demonstrate its application, we then exemplarily use the reactor in the removal of a photolabile protection group during the synthesis of a SARS‐CoV‐2 spike protein glycopeptide. Abstract : The broad application of safely operable photochemistry systems is still limited, mainly due to the scarce availability of affordable models. Here the stepwise planning and assembly of a safe low budget photoreactor is described, using only scrap materials and a commercial LED. As an example, the system is then applied in the removal of a photolabile protection group for the post‐translational modification of synthetic peptides.