Seonamhaeicola maritimus sp. nov., isolated from coastal sediment. Issue 2 (12th November 2019)

• Record Type: Journal Article
• Authors: Cao, Wen-Rui; Lu, De-Chen; Sun, Xun-Ke; Sun, Yuan-Yuan; Saren, Gaowa; Yu, Xin-Ke; Du, Zong-Jun
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 2(2020)
• Page Start: 902
• Page End: 908
• Publication Date: 2019-11-12
• Subjects: Bacteroidetes -- Flavobacteriaceae -- Seonamhaeicola maritimus sp. nov. -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003844
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and orange-pigmented bacterium, designated 1505 T, was isolated from marine sediment that was obtained off the coast of Weihai, PR China. Strain 1505 T was found to grow at 10–35 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, 7.5) and in the presence of 1–4 % (w/v) NaCl (optimum, 2 %). Cells were positive for oxidase and catalase activity. The 16S rRNA gene based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain 1505 T were Seonamhaeicola algicola Gy8 T (97.1 %), Seonamhaeicola marinus B011 T (96.3 %) and Seonamhaeicola aphaedonensis KCTC 32578 T (95.6 %). Based on phylogenomic analysis, the average nucleotide identity values between strain 1505 T and S. algicola Gy8 T, S. marinus B011 T and S. aphaedonensis KCTC 32578 T were 75.9, 76.0 and 77.7 %, respectively; the digital DNA–DNA hybridization values based on the draft genomes between strain 1505 T and S. algicola Gy8 T, S. marinus B011 T and S. aphaedonensis KCTC 32578 T were 20.0, 20.7 and 21.4 %, respectively. Menaquinone-6 (MK-6) was detected as the major respiratory quinone. The dominant cellular fatty acids were iso-C15 : 1 G and C18 : 1 ω9 c . The DNA G+C content of strain 1505 T was 33.3 mol%. The polar lipids included phosphatidylethanolamine, six aminolipids and four unidentified lipids. Based on its phylogenetic and phenotypic characteristics, strain 1505 T is considered to represent a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola maritimus sp. nov. is proposed. The type strain is 1505 T (=KCTC 72528 T =MCCC 1H00389 T ).