Identification, expression and functional analysis of prmt7 in medaka Oryzias latipes. Issue 2 (28th January 2020)

• Record Type: Journal Article
• Authors: Li, Fangqing; Zhu, Huihui; Hou, Mengying; Zhang, Xiaoyi; Li, Zhenzhen; Zhao, Haobin; Zhou, Qingchun; Zhong, Xueping
• Is Part Of: Journal of experimental zoology. Volume 334:Issue 2(2020)
• Page Start: 77
• Page End: 87
• Publication Date: 2020-01-28
• Subjects: embryogenesis -- germ cell development -- medaka -- prmt7; Developmental biology -- Periodicals; Evolution (Biology) -- Periodicals; Molecular evolution -- Periodicals; Zoology -- Periodicals; Evolution, Molecular -- Periodicals; Developmental Biology -- Periodicals; Zoology -- Periodicals; 591
• DOI: 10.1002/jez.b.22927
• Languages: English
• ISSNs: 1552-5007

Abstract

Abstract: Arginine methylation is an important posttranslational modification and catalyzed by a family of protein arginine methyltransferases (PRMTs). PRMT7 is the type III PRMT and produces solely monomethylarginine products. PRMT7 has been found to play important roles in multiple biological processes in mammals. However, the expression pattern and function of Prmt7 remain largely unknown in fish. In this study, we characterized the medaka prmt7 gene and determined its expression pattern and function during embryogenesis and germ cell development. The results showed that the chromosomal location and gene structure of medaka prmt7 were similar to its mammalian orthologs. Comparisons of deduced amino acid sequences indicated that medaka Prmt7 was a homolog of human PRMT7 with two methyltransferase domains. Reverse transcription‐polymerase chain reaction (RT‐PCR) and real time RT‐PCR revealed that medaka prmt7 had maternal origin with continuous and dynamical expression during embryonic development. Whole‐mount in situ hybridization analysis observed that the transcripts of prmt7 were ubiquitous at morula and gastrula stage, and were later riched in the brain and otic vesicles during embryogenesis. In the adult stage, prmt7 messenger RNA was detected in all examined tissues with the high levels in the ovary and testis. The expression of prmt7 in the gonads was restricted to oocytes of the ovary and spermatids/sperm of the testis. Functional analysis showed that knockdown of medaka prmt7 did not reduce the total number of primordial germ cells (PGCs) in vivo but significantly affected PGCs distribution during embryonic development. These results indicate that prmt7 may be involved in germ cell development in medaka. Abstract : Medaka Prmt7 contains two putative methyltransferase domains in tandem, with only one AdoMet‐binding site in the N‐terminal methyltransferase domain. prmt7 had maternal origin and its transcripts were ubiquitous at morula and gastrula stage and were later riched in the brain and otic vesicles. prmt7 messenger RNA was expressed in all examined tissues. The expression of prmt7 in the gonads has restricted to oocytes of the ovary and spermatids/sperm of the testis. Knockdown of prmt7 did not reduce the total number of primordial germ cells (PGCs) in vivo but significantly affected PGCs distribution during medaka embryogenesis. Research Highlights: Medaka prmt7 messenger RNAs were abundant in the gonads and restricted to oocytes and spermatids/sperm. Knockdown of medaka prmt7 significantly affected the distribution of primordial germ cells. These results suggest its roles in germ cell development in medaka.