Putridiphycobacter roseus gen. nov., sp. nov., isolated from Antarctic rotten seaweed. Issue 1 (3rd February 2020)

• Record Type: Journal Article
• Authors: Wang, Xiu-Juan; Xu, Lin; Wang, Ning; Sun, He-Min; Chen, Xiu-Lan; Zhang, Yu-Zhong; Shi, Mei; Zhang, Xi-Ying
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 648
• Page End: 655
• Publication Date: 2020-02-03
• Subjects: the family Crocinitomicaceae -- Antarctic -- gen. nov; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003809
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, pink-pigmented, rod-shaped, non-flagellated, aerobic bacterium, designated strain SM1701 T, was isolated from a rotten seaweed collected off Fildes Peninsula, King George Island, West Antarctica. The strain grew at 4–30 °C, pH 6.0–8.0 and with 0.5–5 % (w/v) NaCl. It hydrolysed gelatin and Tweens (40, 60 and 80), but did not reduce nitrates to nitrites. The major cellular fatty acids of strain SM1701 T were iso-C15 : 0, iso-C15 : 1 G, iso-C16 : 1 G, C16 : 0 and iso-C17 : 0 3-OH. Polar lipids included phosphatidylethanolamine, one unidentified aminolipid, two unidentified glycolipids and one unidentified aminoglycolipid. The major respiratory quinone was MK-7. The genomic DNA G+C content of strain SM1701 T was 34.1 mol%. It showed high 16S rRNA gene sequence similarities to Crocinitomix algicola (93.8 %) and Crocinitomix catalasitica (92.5 %) and less than 91 % sequence similarities to other known members in the family Crocinitomicaceae . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1701 T constituted a distinct lineage within the family Crocinitomicaceae . The phylogenetic trees based on concatenated 261 protein sequences from genome sequences showed that strain SM1701 T occupied a branch separated from those of known genera in the family of Crocinitomicaceae, indicating it may belong to a new genus. On the basis of the polyphasic characterization of strain SM1701 T in this study, it is considered to represent a novel species in a new genus in the family Crocinitomicaceae, for which the name Putridiphycobacter roseus gen. nov., sp. nov. is proposed. The type strain is SM1701 T (=KCTC 62302 T =NBRC 113201 T =CGMCC 1.16510 T ).