Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi). Issue 11 (19th August 2019)

• Record Type: Journal Article
• Authors: Booncharoen, Auttaporn; Visessanguan, Wonnop; Kuncharoen, Nattakorn; Yiamsombut, Supalurk; Santiyanont, Pannita; Mhuantong, Wuttichai; Charoensri, Salisa; Rojsitthisak, Pornchai; Tanasupawat, Somboon
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 11(2019)
• Page Start: 3529
• Page End: 3536
• Publication Date: 2019-08-19
• Subjects: Lentibacillus lipolyticus -- moderately halophile -- shrimp paste -- Ka-pi; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003658
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9 T, was isolated from traditional salted shrimp paste ( Ka-pi ) produced in Samut Sakhon Province, Thailand. This strain grew optimally at 37–40 °C, pH 7.0 and in the presence of 8–16 % (w/v) NaCl. The 16S rRNA gene sequence similarity values between strain SSKP1-9 T and Lentibacillus juripiscarius TISTR 1535 T and Lentibacillus halophilus TISTR 1549 T were 98.7 and 97.2 %, respectively. Based on 16S rRNA gene sequence similarity, strain SSKP1-9 T represents a distinct novel species, as shown by phenotypic traits, DNA–DNA hybridization and average nucleotide identity values. In addition, the whole-cell protein profile confirmed the novelty of the taxon. The genomic DNA G + C content was 44.6 mol%. The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso -diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 . The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9 T represents a novel species of Lentibacillus, for which the name Lentibacillus lipolyticus sp. nov. is proposed. The type strain is SSKP1-9 T (=JCM 32625 T =TISTR 2597 T ).