Solirhodobacter olei gen. nov., sp. nov., a nonphotosynthetic bacterium isolated from oil-contaminated soil. Issue 1 (3rd February 2020)

• Record Type: Journal Article
• Authors: Chu, Cuiwei; Liu, Bin; Lian, Ziyi; Zheng, Huiying; Chen, Can; Yue, Zonghao; Li, Lili; Sun, Zhongke
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 582
• Page End: 588
• Publication Date: 2020-02-03
• Subjects: Solirhodobacter olei -- Solirhodobacter -- Rhodobacteraceae -- nonphotosynthetic bacterium -- oil-contaminated soil; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003795
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A nonphotosynthetic, Gram-stain-negative, rod-shaped and motile strain, designated Pet-1 T, was isolated from oil-contaminated soil collected from Daqing oil field in China. Optimal growth occurred at 37 °C, pH 5.5 and in 1 % (w/v) NaCl. Q-10 was the sole respiratory quinone. The most abundant fatty acid was C18 : 1 ɷ7 c /C18 : 1 ɷ6 c (67.4 %). The major polar lipids were phosphatidylglycerol, aminolipid, phosphatidylethanolaine, phosphatidycholine, two unidentified lipids and two unidentified phospholipids. The genomic DNA G+C content was 69.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that Pet-1 T shared the highest similarity (95.1 %) to Rhodobacter vinaykumarii DSM 18714 T, followed by Sinorhodobacter populi sk2b1 T (95.0 %) and Haematobacter massiliensis CCUG 47968 T (95.0 %). In the phylogenetic tree, strain Pet-1 T formed a separate branch from the closely related genera Rhodobacter, Pararhodobacter, Defluviimonas and Rhodovulum within the family Rhodobacteraceae . Based on the data from the current polyphasic study, it is proposed that the isolate is a novel species of a novel genus within the family Rhodobacteraceae, with the name Solirhodobacter olei gen. nov., sp. nov. The type strain of the type species is Pet-1 T (=KCTC 72074 T =CCTCC AB 2018368 T ).