Dyadobacter psychrotolerans sp. nov. and Dyadobacter frigoris sp. nov., two novel psychrotolerant members of the family Cytophagaceae isolated from Arctic soil. Issue 1 (3rd February 2020)

• Record Type: Journal Article
• Authors: Chaudhary, Dhiraj Kumar; Dahal, Ram Hari; Kim, Jaisoo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 569
• Page End: 575
• Publication Date: 2020-02-03
• Subjects: Dyadobacter psychrotolerans -- Dyadobacter frigoris -- Cytophagaceae -- Arctic soil; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003796
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Four strains of bacteria designated as AR-3-6 T, AT-3–1, AR-3-8 T and AR-3–15 were isolated from Arctic soil. Cells were aerobic, Gram-staining-negative, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. Flexirubin-type pigments were present in all strains. All strains tolerated 2 % of NaCl and were psychrotolerant. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that these strains formed a lineage within the family Cytophagaceae that were distinct from various members of the genus Dyadobacter . The closest member of strain AR-3-6 T was D. koreensis DSM 19938 T (97.2 % sequence similarity) and AR-3-8 T was D. hamtensis HHS 11 T (97.9 %). The predominant respiratory quinone was menaqinone-7. The major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6 c ), iso-C15 : 0, C16 : 1 ω5 c and iso-C17 : 0 3-OH. The DNA G+C content of strains ranges from 40.1 to 42.1 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, both strains AR-3-6 T and AR-3-8 T represent a novel member in the genus Dyadobacter, for which the name Dyadobacter psychrotolerans sp. nov. and Dyadobacter frigoris sp. nov. are proposed, respectively. The type strain of Dyadobacter psychrotolerans is AR-3-6 T (=KEMB 9005–743 T =KACC 21172 T =NBRC 113790 T ) and type strain of Dyadobacter frigoris is AR-3-8 T (=KEMB 9005–744 T = KACC 21173 T =NBRC 113791 T ).