Luteimonas lumbrici sp. nov., a novel bacterium isolated from wormcast. Issue 1 (3rd February 2020)

• Record Type: Journal Article
• Authors: Cha, Qi-Yan; Zhou, Xing-Kui; Zhang, Xiao-Fei; Li, Miao; Wei, Yu-Qian; Zhang, Ti-Kun; Qin, Shi-Chun; Liu, Zi-Yi; Wang, Xue-Jian; Liu, Jian-Jin; Zhu, Ming-Liang; Mo, Ming-He
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 604
• Page End: 610
• Publication Date: 2020-02-03
• Subjects: Luteimonas lumbrici sp. nov. -- 16S rRNA gene -- polyphasic taxonomic analysis -- wormcast; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003799
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, yellow-green bacterium, designated 1.1416 T, was isolated from wormcast of Eisenia foetida . The strain was non-motile, rod-shaped, and grew optimally on NA medium at 30 °C, pH 7.0 and with 0 % (w/v) NaCl. On the basis of the 16S rRNA gene sequence and phylogenetic analysis, 1.1416 T showed the highest degree of 16S rRNA gene sequence similarity to Luteimonas arsenica 26-35 T (96.2 %), followed by Luteimonas lutimaris G3 T (96.1 %). The respiratory quinone of 1.1416 T was ubiquinone-8 (Q-8), and its major cellular fatty acids were iso-C15 : 0 (39.8 %), summed feature 9 (iso-C17 : 1 ω9 c or C16 : 0 10-methyl) (18.6 %). The major polar lipids of 1.1416 T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and six unidentified phospholipids. The genomic DNA G+C content of 1.1416 T was 71.0 mol%. According to the results of the phenotypic and chemotaxonomic phylogenetic analyses, strain 1.1416 T represents a novel species of the genus Luteimonas, for which the name Luteimonas lumbrici sp. nov. is proposed, with strain 1.1416 T (=KCTC 62979 T =CCTCC AB 2018348 T ) as the type strain.