Bacillus natronophilus sp. nov., an alkaliphilic bacterium isolated from a soda lake. Issue 1 (3rd February 2020)

• Record Type: Journal Article
• Authors: Menes, Rodolfo Javier; Machin, Eliana Valentina; Iriarte, Andrés; Langleib, Mauricio
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 562
• Page End: 568
• Publication Date: 2020-02-03
• Subjects: Bacillus natronophilus sp. nov -- alkaliphile -- moderately halophilic -- soda lake -- Bacillus sp; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003792
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30 T ) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30 T was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12–43 °C (optimum, 25–30 °C), at pH 8.0–12 (optimum, pH 9.5–10) and at salinities of 0.5–15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained meso -diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9 mol%. The predominant menaquinone was MK-7 and the major fatty acids (>10 %) comprised anteiso-C15 : 0, iso-C15 : 0, and anteiso-C17 : 0 . Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30 T to the genus Bacillus and showed the highest similarities to Bacillus populi FJAT-45347 T (96.4 %) and Bacillus aurantiacus K1-5 T (96.2 %). Based on the data from the current polyphasic study, M30 T represents a novel species of the genus Bacillus, for which the name Bacillus natronophilus sp. nov. is proposed. The type strain is M30 T (=JCM 32118 T =CGMCC 1.16739 T =MCC 3010 T ).