Emticicia agri sp. nov., a novel member of the family Cytophagaceae. Issue 11 (16th August 2019)

• Record Type: Journal Article
• Authors: Ten, Leonid N.; Li, Weilan; Ha, Aeri; Kim, Myung Kyum; Rooney, Alejandro P.; Jung, Hee-Young
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 11(2019)
• Page Start: 3492
• Page End: 3499
• Publication Date: 2019-08-16
• Subjects: Emticicia -- Cytophagaceae -- soil bacteria; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003651
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A bacterial strain, 17J42-9 T, was isolated from a soil sample collected on Jeju Island, Republic of Korea. Cells were observed to be Gram-stain negative and rod-shaped. Colonies were observed to be orange in colour on R2A agar. Analysis of 16S rRNA gene sequences showed that high levels of 16S rRNA sequence similarity were shared between 17J42-9 T and Emticicia fontis IMCC1731 T (98.2 %), Emticicia ginsengisoli Gsoil 085 T (98.2 %) and Emticicia soli ZZ-4 T (97.8 %). Growth of strain 17J42-9 T was observed at 10–37 °C, pH 6.0–8.5 and in the presence of 0–0.5 % NaCl. The genomic G+C content was calculated to be 38.6 mol%. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as summed feature 3 (C16 : 1 ω 7 c and/or C16 : 1 ω 6 c ) (34.1 %), C15 : 0 iso (23.4 %) and C17 : 0 iso 3-OH (10.8 %). The major polar lipids were found to be phosphatidylethanolamine, two unidentified aminolipids and an unidentified lipid. The phenotypic and chemotaxonomic data support the affiliation of strain 17J42-9 T with the genus Emticicia . However, the DNA–DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 46 %. The results of 16S rRNA gene sequence similarity analysis, DNA–DNA hybridization analysis and the observed differentiating phenotypic properties from other closely related taxa clearly indicate that strain 17J42-9 T represents a novel species in the genus Emticicia, for which the name Emticicia agri sp. nov. is proposed. The type strain is 17J42-9 T (=KCTC 62270 T =JCM 33056 T ).