Ramlibacter humi sp. nov., isolated from tropical forest soil. Issue 11 (21st August 2019)

• Record Type: Journal Article
• Authors: Zhang, Xian-Jiao; Feng, Guang-Da; Yao, Qing; Wang, Yong-Hong; Yang, Song-Zhen; Zhu, Hong-Hui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 11(2019)
• Page Start: 3460
• Page End: 3464
• Publication Date: 2019-08-21
• Subjects: Ramlibacter humi -- Ramlibacter -- tropical forest soil -- ANI -- dDDH -- draft genome sequence; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003641
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1 T, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15–37 °C and pH 6.0–8.0 without NaCl. The 16S rRNA gene sequence analyses showed that strain 18x22-1 T was closely related to Ramlibacter tataouinensis DSM 14655 T (98.5 %), followed by Ramlibacter henchirensis DSM 14656 T (97.9 %) and other Ramlibacter species and formed a stable cluster with R. tataouinensis DSM 14655 T, R. henchirensis DSM 14656 T, Ramlibacter solisilvae JCM 19319 T and Ramlibacter rhizophilu s CCTCC AB 2015357 T . Results of chemotaxonomic analyses showed that ubiquinone-8 (Q-8) was the major respiratory quinone, and the major fatty acids (>10 % of the total amounts) were C16 : 0 and C17 : 0 cyclo. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminopholipids and four unidentified phospholipids. The draft genome sequence was 4.47 Mb long with a G+C content of 68.9 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain 18x22-1 T and four closely related type strains were in the range of 79.3–82.3 % and 21.9–25.1 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses supported that strain 18x22-1 T represents a novel species of the genus Ramlibacter, for which the name Ramlibacter humi sp. nov. is proposed. The type strain is 18x22-1 T (=GDMCC 1.1584 T =KCTC 52922 T ).