Leucothrix sargassi sp. nov., isolated from a marine alga [Sargassum natans (L.) Gaillon]. Issue 12 (5th September 2019)

• Record Type: Journal Article
• Authors: Liu, Tingwei; Zhang, Yao; Zhang, Xuan; Zhou, Lixin; Meng, Chunyu; Zhou, Chenyan; Jing, Changqin; Zhu, Wuling; Wang, Yan
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 12(2019)
• Page Start: 3857
• Page End: 3862
• Publication Date: 2019-09-05
• Subjects: Leucothrix -- 16S rRNA gene -- 120 ubiquitous single-copy proteins -- average nucleotide identity; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003694
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel bacterial strain, C3212 T, was isolated from a marine alga collected from the sea shore of Yantai, China. The strain was Gram-stain-negative, rod-shaped, aerobic, non-motile, and oxidase- and catalase-positive. Growth was observed at 8–37 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 1.0–7.0 % (w/v) NaCl (optimum, 4.0 %). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain C3212 T consisted of diphosphatidylglycerol (cardiolipin), phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified polar lipid. The major fatty acids were C16 : 1 ω 6c and/or C16 : 1 ω 7c, and C18 : 1 ω 6c and/or C18 : 1 ω 7c . The DNA G+C content of strain C3212 T was 44.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Leucothrix pacifica XH122 T, Leucothrix arctica IMCC 9719 T and Leucothrix mucor DSM 2157 T with similarities of 98.0, 97.5 and 94.3 %, respectively. Estimated DNA–DNA hybridization values were 14.2, 20.7 and 13.9 % between strain C3212 T and L. pacifica XH122 T, L. arctica IMCC 9719 T and L. mucor DSM 2157 T, respectively. Phenotypic, phylogenetic and genomic analyses revealed that strain C3212 T represents a novel species of the genus Leucothrix, for which the name Leucothrix sargassi sp. nov. is proposed. The type strain is C3212 T (=MCCC 1K03600 T =KCTC 72121 T ).