Isolation of Bordetella species from unusual infection sites. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Almuzara, Marisa; Barberis, Claudia; Traglia, Germán; Sly, Gabriela; Procopio, Adriana; Vilches, Viviana; Ramirez, Maria Soledad; Famiglietti, Angela; Vay, Carlos
• Is Part Of: JMM case reports. Volume 2:Issue 2(2015)
• Publication Date: 2015-04
• Subjects: Bordetella infections -- species‐dependent antibiotic susceptibility -- unusual infection sites; Medical microbiology -- Periodicals; 616.9041
• DOI: 10.1099/jmmcr.0.000029
• Languages: English
• ISSNs: 2053-3721

Abstract

Abstract : Introduction: Bordetella hinzii has been isolated mainly from respiratory specimens and from blood of immunocompromised patients, and Bordetella trematum from ear infections or leg, arm and ankle wounds and from diabetic foot ulcers. Bordetella holmesii is instead associated with bactaeremia in young adults, mostly with underlying conditions. Only three septic arthritis cases due to this species have been described in the literature. Case presentation: Herein we describe four cases of infections due to Bordetella species that have been recovered from unusual infection sites: two cases of B. hinzii infections, one recovered from the urine of a patient with chronic prostatitis and the other from a liver cyst in an immunocompetent patient; one B. trematum case from a bone biopsy of a patient with chronic osteomyelitis of the hip; and one B. holmesii case isolated from the joint fluid of an immunocompetent patient with diagnosed septic arthritis. The organisms were identified using standard biochemical tests, by API 20 NE version 6.0, by automated system VITEK 2, by mass spectrometry using the Bruker Daltonics MicroFlex LT spectrometer with MALDI Biotyper 3.1, and by PCR amplification of 16S rRNA. Antibiotic susceptibility testing was performed using the VITEK 2 system, except for B. holmesii, for which the epsilometric method (Etest technique; bioMérieux) was used. Conclusion: We highlight the importance of isolating Bordetella species from severe infections and unusual sites, and also of combining both phenotypic and genotypic methods for definitive identification.