Calcium‐Sensing Receptors in Chondrocytes and Osteoblasts Are Required for Callus Maturation and Fracture Healing in Mice. (18th October 2019)

• Record Type: Journal Article
• Authors: Cheng, Zhiqiang; Li, Alfred; Tu, Chia‐Ling; Maria, Christian Santa; Szeto, Nicholas; Herberger, Amanda; Chen, Tsui‐Hua; Song, Fuqing; Wang, Jiali; Liu, Xiaodong; Shoback, Dolores M; Chang, Wenhan
• Is Part Of: Journal of bone and mineral research. Volume 35:Number 1(2020)
• Page Start: 143
• Page End: 154
• Publication Date: 2019-10-18
• Subjects: CARTILAGE -- CaSR KNOCKOUT -- CHONDROCYTE -- CHONDROGENESIS -- ENDOCHONDRAL BONE FORMATION -- FRACTURE HEALING -- OSTEOBLAST -- OSTEOGENESIS -- UNFIXED FRACTURE; Bones -- Metabolism -- Periodicals; Mineral metabolism -- Periodicals; 612.392
• DOI: 10.1002/jbmr.3864
• Languages: English
• ISSNs: 0884-0431

Abstract

ABSTRACT: Calcium and its putative receptor (CaSR) control skeletal development by pacing chondrocyte differentiation and mediating osteoblast (OB) function during endochondral bone formation—an essential process recapitulated during fracture repair. Here, we delineated the role of the CaSR in mediating transition of callus chondrocytes into the OB lineage and subsequent bone formation at fracture sites and explored targeting CaSRs pharmacologically to enhance fracture repair. In chondrocytes cultured from soft calluses at a closed, unfixed fracture site, extracellular [Ca 2+ ] and the allosteric CaSR agonist (NPS‐R568) promoted terminal differentiation of resident cells and the attainment of an osteoblastic phenotype. Knockout (KO) of the Casr gene in chondrocytes lengthened the chondrogenic phase of fracture repair by increasing cell proliferation in soft calluses but retarded subsequent osteogenic activity in hard calluses. Tracing growth plate (GP) and callus chondrocytes that express Rosa26‐tdTomato showed reduced chondrocyte transition into OBs (by >80%) in the spongiosa of the metaphysis and in hard calluses. In addition, KO of the Casr gene specifically in mature OBs suppressed osteogenic activity and mineralizing function in bony calluses. Importantly, in experiments using PTH (1‐34) to enhance fracture healing, co‐injection of NPS‐R568 not only normalized the hypercalcemic side effects of intermittent PTH (1‐34) treatment in mice but also produced synergistic osteoanabolic effects in calluses. These data indicate a functional role of CaSR in mediating chondrogenesis and osteogenesis in the fracture callus and the potential of CaSR agonism to facilitate fracture repair. © 2019 American Society for Bone and Mineral Research.