MiR-504 Promoted Gastric Cancer Cell Proliferation and Inhibited Cell Apoptosis by Targeting RBM4. (7th June 2021)

• Record Type: Journal Article
• Authors: Zhang, Yi; Yong, Hongmei; Fu, Jing; Gao, Guangyi; Shi, Huichang; Zhou, Xueyi; Fu, Mingsheng
• Other Names: Lu Xiao-Jie Academic Editor.
• Is Part Of: Journal of immunology research. Volume 2021(2021)
• Publication Date: 2021-06-07
• Subjects: Immunology -- Periodicals; Immunology -- Research -- Periodicals; 616.07905
• DOI: 10.1155/2021/5555950
• Languages: English
• ISSNs: 2314-8861

Abstract

Abstract : Background . The purpose of this study was to explore the role and underlying mechanism of miR-504 and RBM4 in gastric cancer. Methods . The qRT-PCR or Western blot was performed to determine the expressions of miR-504 and RBM4 in the gastric cancer tissues and normal tissues. Human SGC-7901 cells were transfected with miR-504 mimic/inhibitor or pcDNA-RBM4. Cell proliferation and cell apoptosis were assessed by colony formation assay and flow cytometry, respectively. Luciferase reporter gene assays were used to investigate interactions between miR-504 and RBM4 in SGC-7901 cells. Results . The relative expression of miR-504 was significantly upregulated in the gastric cancer group (n = 25 ) than in the paired normal group (n = 25 ), but the relative RBM4 expression was remarkably downregulated in the gastric tumor group, compared with the normal group. Additionally, miR-504 overexpression increased the viability of gastric cancer cells. Moreover, RBM4 is a functional target of miR-504 in gastric cancer cells. miR-504 was further confirmed to promote SGC-7901 cell proliferation and inhibit cell apoptosis by downregulation RBM4 in vitro. Conclusions . miR-504 promotes gastric cancer cell proliferation and inhibits cell apoptosis by targeting RBM4, and this provides a potential diagnostic biomarker and treatment for patients with gastric cancer.