A Protocol for Measurement of Noncoding RNA in Human Serum. (1st July 2012)

• Record Type: Journal Article
• Authors: Taylor, Caroline J.; Satoor, Sarang N.; Ranjan, Amaresh K.; Pereira e Cotta, Maria V.; Joglekar, Mugdha V.
• Other Names: Hardikar Anandwardhan Academic Editor.
• Is Part Of: Experimental diabetes research. Volume 2012(2012)
• Publication Date: 2012-07-01
• Subjects: Diabetes -- Research -- Periodicals; Diabetes -- Research; Periodicals; 616.462
• DOI: 10.1155/2012/168368
• Languages: English
• ISSNs: 1687-5214

Abstract

Abstract : MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.