Variation of antibiotic resistome during commercial livestock manure composting. (March 2020)

• Record Type: Journal Article
• Authors: Zhang, Min; He, Liang-Ying; Liu, You-Sheng; Zhao, Jian-Liang; Zhang, Jin-Na; Chen, Jun; Zhang, Qian-Qian; Ying, Guang-Guo
• Is Part Of: Environment international. Volume 136(2020)
• Publication Date: 2020-03
• Subjects: Antibiotic resistance genes -- Livestock manure -- Composting -- Microbial community -- Pathogens -- Antibiotic resistome; Environmental protection -- Periodicals; Environmental health -- Periodicals; Environmental monitoring -- Periodicals; Environmental Monitoring -- Periodicals; Environnement -- Protection -- Périodiques; Hygiène du milieu -- Périodiques; Environnement -- Surveillance -- Périodiques; Environmental health; Environmental monitoring; Environmental protection; Periodicals; 333.705
• DOI: 10.1016/j.envint.2020.105458
• Languages: English
• ISSNs: 0160-4120

Abstract

Graphical abstract: Highlights: We investigated antibiotic resistome during manure composting by metagenomics. Moisture was the key environmental factor over composting. Composting significantly reduced the relative abundance of ARGs and MGEs. Actinobacteria was the primary potential host of ARGs over composting. Bacterial community and BMRGs profiles structured the antibiotic resistome. Abstract: Composting has been widely used to turn livestock manure into organic fertilizer. However, livestock manure contains various contaminants including antibiotics and antibiotic resistance genes (ARGs). Here we investigated the variation of antibiotic resistome and its influencing factors during a commercial livestock manure composting. The results showed that composting could effectively reduce the relative abundance of ARGs and mobile genic elements (MGEs). As the dominant phylum in the composting samples, the key potential bacterial host of ARGs were Actinobacteria such as Leucobacter, Mycobacterium and Thermomonosporaceae unclassified. Meanwhile, Legionella pneumophila, Staphylococcus saprophyticus, Haemophilus ducreyi and Siccibacter turicensis may be the key potential pathogenic host of ARGs because of their co-occurrence with ARG subtypes. Redundancy analysis showed that the dissipation of ARGs during composting was linked to various environmental factors such as moisture. Bacterial succession as well as profile of biocide and metal resistance genes (BMRGs) were the determinants which constructed the antibiotic resistome during manure composting. However, the residues of ARGs and pathogens in compost products may still pose risks to human and crops after fertilization.