Investigation of early antibiotic use in pediatric patients with acute respiratory infections by high‐performance liquid chromatography. (12th November 2019)

• Record Type: Journal Article
• Authors: Van Toi, Pham; Doan, Khanh V.; Minh, Ngo Ngọc Quang; Phuong, Pham Nguyen; de Jong, Menno D.; van Doorn, H. Rogier; Pouplin, Thomas
• Is Part Of: Biomedical chromatography. Volume 34:Number 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-11-12
• Subjects: high‐performance liquid chromatography -- β‐Lactams -- antibiotic misuse -- pediatric patients -- urine; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4699
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: In this study, we developed and validated two reliable high‐performance liquid chromatography (HPLC) methods for the qualitative detection of six oral β ‐lactams, which are commonly used in pediatric patients with acute respiratory infections (ARIs). Two distinct reverse‐phase chromatographic separations of six β ‐lactams were obtained. Four β ‐lactams (cefadroxil, cephalexin, cefaclor and cefixime) in urine were separated using a gradient program with a mobile phase consisting of K2 HPO4 buffer (20 mm, pH 2.8) and acetonitrile on a LichroCART 250 × 4.6 mm, Purospher STAR C18 end‐capped (5 μm) column. Two remained β ‐lactams (amoxicillin and cefuroxime) were analyzed using a gradient elution with the mobile phase containing K2 HPO4 buffer (20 mm, pH 3.0) and acetonitrile on a LichroCart ® Purospher Star C8 end‐capped column (5 μm, 125 × 4.6 mm). Good linearity within the range of 0.3–30 μg/ml for cefadroxil, cephalexin, cefaclor and cefixime, and 0.2–20 μg/ml for amoxicillin and cefuroxime, was attained. The precisions were <14%. The accuracies ranged from 85.87 to 102.8%. The two validated methods were then applied to determine these six antibiotics in 553 urine samples of pediatric patients with ARIs. As a result, 32.2% were positive with one or more of six tested β ‐lactams. Cefixime was the most commonly detected agent, accounting for 9.8% of enrolled patients.