Needleless emulsion electrospinning for the regulated delivery of susceptible proteins. (29th November 2017)

• Record Type: Journal Article
• Authors: Buzgo, Matej; Filova, Eva; Staffa, Andrea Mickova; Rampichova, Michala; Doupnik, Miroslav; Vocetkova, Karolina; Lukasova, Vera; Kolcun, Radka; Lukas, David; Necas, Alois; Amler, Evzen
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 12:Number 3(2018)
• Page Start: 583
• Page End: 597
• Publication Date: 2017-11-29
• Subjects: emulsion electrospinning -- growth factor -- needleless core/shell electrospinning -- Pluronic F‐68 -- time‐regulated release; Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.2474
• Languages: English
• ISSNs: 1932-6254

Abstract

Abstract: In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly‐ε‐caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F‐68 (PF‐68). The PCL‐PF‐68 nanofibres show a time‐regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5‐(4, 6‐dichlorotriazinyl) aminofluorescein ‐PF‐68, horseradish peroxidase, Tetramethylrhodamine‐dextran, insulin growth factor‐I, transforming growth factor‐β and basic fibroblast growth factor]. In addition, the PF‐68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue‐engineering applications.