Classical Hodgkin lymphoma cells may promote an IL-17-enriched microenvironment. Issue 14 (6th December 2019)

• Record Type: Journal Article
• Authors: Ferrarini, Isacco; Rigo, Antonella; Zamò, Alberto; Vinante, Fabrizio
• Is Part Of: Leukemia & lymphoma. Volume 60:Issue 14(2019)
• Page Start: 3395
• Page End: 3405
• Publication Date: 2019-12-06
• Subjects: Interleukin 17 -- classical Hodgkin lymphoma -- microenvironment -- T helper lymphocytes -- CD30; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.1080/10428194.2019.1636983
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract: In classical Hodgkin lymphoma (cHL), the significance of the interplay between Hodgkin and Reed–Sternberg cells (HRS) and reactive T cells remains poorly defined. By immunohistochemistry on bioptic cHL specimens, we found that HRS and surrounding T lymphocytes stained positive for IL-17 in 40% of cases. IL-17 was detectable in a similar proportion of patients' sera and correlated with disease burden. Supernatants of KM-H2 and HDLM-2 cHL cell lines guided preferential chemotaxis of CCR6 + T lymphocytes. Coculture of cHL cell lines with PBMC promoted the enrichment of Th17 lymphocytes and Foxp3 + /IL-17 + cells, whereas T regulatory cells slightly decreased. Soluble CD30 downmodulated membrane CD30 expression on T cells and contributed to their polarization shift by stimulating IL-17 production and reducing IFN-γ synthesis. Thus, HRS and a number of reactive CD4 + T cells, attracted by tumor-secreted chemokines, produce an IL-17 tumor-shaped inflammatory milieu in a cHL subset.