Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus). Issue 4 (2nd October 2019)

• Record Type: Journal Article
• Authors: An, Hyejin; Jo, Ick-Hyun; Oh, Youn-Lee; Jang, Kab-Yeul; Kong, Won-Sik; Sung, Jwa-Kyung; So, Yoon-Sup; Chung, Jong-Wook
• Is Part Of: Mycobiology. Volume 47:Issue 4(2019)
• Page Start: 527
• Page End: 532
• Publication Date: 2019-10-02
• Subjects: Button mushroom -- cluster analysis -- genetic diversity -- simple sequence repeat marker; Mycology -- Periodicals; Mycology -- Korea -- Periodicals; Fungi -- Periodicals; Fungi -- Korea -- Periodicals; Fungi; Mycology; Korea; Mycology -- Periodicals; Fungi -- Periodicals; Periodicals; Electronic journals; 579.5
• DOI: 10.1080/12298093.2019.1667131
• Languages: English
• ISSNs: 1229-8093

Abstract

Abstract: We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom ( Agaricus bisporus ), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (MAF ) and the average number of alleles (NA ) were 0.50 and 5.47, respectively. The average number of genotypes (NG ), observed heterozygosity (HO ), expected heterozygosity (HE ), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that MAF was negatively correlated with NG (−0.683), NA (−0.600), HO (−0.584), and PIC (−0.941). NG, NA, HO, and PIC were positively correlated with other polymorphic parameters except for MAF . UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.