A minimal hybridization chain reaction (HCR) system using peptide nucleic acids. Issue 23 (19th May 2021)

• Record Type: Journal Article
• Authors: Kim, Ki Tae; Angerani, Simona; Winssinger, Nicolas
• Is Part Of: Chemical science. Volume 12:Issue 23(2021)
• Page Start: 8218
• Page End: 8223
• Publication Date: 2021-05-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1sc01269j
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : A minimal peptide nucleic acid (PNA) HCR system based on a 5-mer stem and 5-mer loop/toehold hairpins was developed. The system was applied to the detection of a cancer biomarker on the surface of living cells. Abstract : The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of γ-peg-modified PNA was found to be beneficial.