Cultivation of Nostoc sp. LS04 in municipal wastewater for biodiesel production and their deoiled biomass cellular extracts as biostimulants for Lactuca sativa growth improvement. (October 2021)

• Record Type: Journal Article
• Authors: Silambarasan, Sivagnanam; Logeswari, Peter; Sivaramakrishnan, Ramachandran; Kamaraj, Balu; Lan Chi, Nguyen Thuy; Cornejo, Pablo
• Is Part Of: Chemosphere. Volume 280(2021)
• Publication Date: 2021-10
• Subjects: Nostoc sp. LS04 -- Municipal wastewater -- Nutrients removal -- Biodiesel -- Biostimulants -- Lettuce; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.130644
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: In this study, seven different cyanobacteria (LS01-LS07) were isolated from paddy field water and among them, the isolate LS04 was able to grow well on municipal wastewater. The LS04 isolate was identified as Nostoc sp. (designated as Nostoc sp. LS04) based on 16S rRNA gene sequence analysis. Strain LS04 grew well in 75% wastewater and had the greatest nutrients removal efficiency (81.02–95.17%). Strain LS04 obtained the higher biomass (1.31 ± 0.08 g L −1 ) and productivity of 131.33 ± 8.08 mg L −1 d −1 . The lipid content and productivity of LS04 were 14.85 ± 0.86% (dry cell weight) and 19.46 ± 0.05 mg L −1 d −1, respectively. The high proportion of C16–C18 fatty acids found in the lipids of LS04 indicated the high suitability for biodiesel production. In addition, Nostoc sp. LS04 cellular extracts were potentially used as a biostimulant for Lactuca sativa cultivation. The foliar application of 60% LS04 cellular extracts showed the maximum shoot length, root length, fresh biomass, dry biomass, Chl a, Chl b and carotenoids in lettuce plants compared to control plants. Similarly, 60% of LS04 cellular extracts treatment improved the concentrations of macro and micronutrients, and biochemical compounds in the leaves. Therefore, these results reveal that the Nostoc sp. LS04 is a promising candidate for the nutrients removal from wastewater and their biomass is a potential resource for biodiesel production and biostimulant for sustainable crop production. Graphical abstract: Image 1 Highlights: Nostoc sp. LS04 efficiently removed the pollutants in municipal wastewater. 1.31 g L −1 of biomass and 14.85% w/w of lipid content were obtained. Strain LS04 lipid had desirable quality for biodiesel production. Nostoc sp. LS04 cellular extract was successfully used as biostimulant.