Comparison of point-of-care test and enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies in the diagnosis of human schistosomiasis japonica. (June 2021)

• Record Type: Journal Article
• Authors: Rodpai, Rutchanee; Sadaow, Lakkhana; Boonroumkaew, Patcharaporn; Phupiewkham, Weeraya; Thanchomnang, Tongjit; Limpanont, Yanin; Chusongsang, Phiraphol; Sanpool, Oranuch; Ohmae, Hiroshi; Yamasaki, Hiroshi; Intapan, Pewpan M.; Maleewong, Wanchai
• Is Part Of: International journal of infectious diseases. Volume 107(2021)
• Page Start: 47
• Page End: 52
• Publication Date: 2021-06
• Subjects: Schistosoma japonicum -- Schistosomiasis japonica -- Adult somatic antigen -- Serodiagnosis -- Immunochromatographic test kit -- Point-of-care test; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2021.04.039
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: Rapid detection of anti- Schistosoma japonicum IgG antibodies in human sera. Development of adult worm extracted-based immunochromatographic test. Usefulness for the point-of-care serodiagnosis of schistosomiasis japonica. Sensitive and specific tools for screening and detections of infection. Abstract: Objective: Schistosomiasis japonica is an important helminthic disease in Asia. Sensitive and accurate diagnostic tools are indispensable for clinical diagnosis, screening infection and monitoring its control. In this study, we developed an immunochromatographic test ( Sj -ICT) to detect anti- Schistosoma japonicum immunoglobulin G antibodies in human sera. Methods: Somatic extract from adult S. japonicum was used as an antigen. The Sj -ICT was developed and optimized as a point-of-care test. All 214 human serum samples were evaluated for diagnostic usefulness and comparison with an enzyme-linked immunosorbent assay (ELISA). Results: The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy of the Sj -ICT were 90.8%, 87.9%, 86.4%, 91.9% and 89.3%, respectively. For ELISA the values were respectively 91.8%, 87.9%, 86.5%, 92.7% and 89.7%. The concordance between both methods was 86.4 % (Cohen's kappa value = 0.729). Conclusions: The immunochromatographic test kit developed can support clinical diagnosis and large-scale surveys in endemic areas without requiring additional facilities or ancillary supplies.