CELL CYCLE SEITCH 52 regulates tillering by interacting with LATERAL SUPPRESSOR in non-heading Chinese cabbage. (August 2021)

• Record Type: Journal Article
• Authors: Guo, Mingliang; Long, Yan; Xu, Lanlan; Zhang, Wei; Liu, Tongkun; Zhang, Changwei; Hou, Xilin; Li, Ying
• Is Part Of: Plant science. Volume 309(2021)
• Publication Date: 2021-08
• Subjects: Non-heading Chinese cabbage -- Tiller -- Virus-induced gene silencing -- LATERAL SUPPRESSOR -- CELL CYCLE SEITCH 52; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2021.110934
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: BcLAS positively regulates the tillering of non-heading Chinese cabbage at the vegetative growth stage. BcCCS52 inhibits the tillering of of non-heading Chinese cabbage at the vegetative growth stage. BcCCS52 interacts with BcLAS. Abstract: With the discovery of essential genes regulating tillering, such as MONOCULM 1 ( MOC1 ) in rice and LATERAL SUPPRESSOR ( LAS in Arabidopsis, LS in tomato), research on tillering mechanisms has made great progress; however, the study of tillering in non-heading Chinese cabbage (NHCC) is rare. Here, we report that BcLAS, as a member of the GRAS family, plays an important role in the tillering of NHCC during its vegetative growth. BcLAS was almost not expressed in other examed parts except leaf axils throughout life. When the expression of BcLAS was silenced utilizing virus-induced gene silencing (VIGS) technology, we found that the tiller number of 'Maertou' decreased sharply. In 'Suzhouqing', overexpression of BcLAS significantly promoted tillering. BcCCS52, the orthologue to CELL CYCLE SEITCH 52 (CCS52), interacts with BcLAS. Downregulation of the expression of BcCCS52 promoted tillering of 'Suzhouqing'; therefore, we conclude that BcCCS52 plays a negative role in tillering regulation. Our findings reveal the tillering regulation mechanism of NHCCs at the vegetative stage and report an orthologue of CCS52 regulating tillering in NHCC.