Global transcriptome profiling reveals antagonizing response of head kidney of juvenile common carp exposed to glyphosate. (October 2021)

• Record Type: Journal Article
• Authors: Liu, Jing-Bo; Chen, Kai; Liu, Tian-Bin; Wang, Zhen-Yong; Wang, Lin
• Is Part Of: Chemosphere. Volume 280(2021)
• Publication Date: 2021-10
• Subjects: Glyphosate -- Head kidney -- Transcriptome profiling -- Antagonizing response -- Apoptosis -- Cell proliferation; DCFH-DA 7′-dichloro-dihydro-fluorescein diacetates -- DEGs differentially expressed genes -- ECM extracellular matrix -- GLY glyphosate -- GO Gene Ontology -- IL-1β interleukin-1β -- KEGG Kyoto Encyclopedia of Genes and Genomes -- MAPK mitogen-activated protein kinase -- MDA malonaldehyde -- PVDF polyvinylidene fluoride -- qRT-PCR Quantitative reverse-transcriptional polymerase chain reaction -- ROS reactive oxygen species -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- TGF-β1 transforming growth factor-β1 -- TNF tumor necrosis factor; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.130823
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Glyphosate (GLY) frequently detected in various water bodies has imposed a serious risk on fish. Head kidney of fish is an important defense organ, playing a vital part in antagonizing exogenous hazardous matter. The objective of this study was to characterize toxic mechanisms of GLY in head kidney of common carp based on transcriptome profiling. After 45-days exposure of GLY at environmentally relevant concentrations, juvenile common carp were used as experimental subjects to analyze how the head kidney responded to GLY. The transcriptome profiling identified 1381 different expressed genes (DEGs) between the control and exposure groups (5 and 50 mg/L). Functional analysis of DEGs substantiated over-representative pathways mainly involving cellular stress responses, cell proliferation and turnover, apoptosis, lipid metabolism, and innate immune processes in both treated groups compared with the control group. Predicted network of gene regulation indicated that GLY-induced tp53 played a vital role in linking a battery of signals. Furthermore, the expression of 10 candidate genes by qRT-PCR aligned with transcriptional profiling. In addition, western blotting analysis confirmed that GLY-induced apoptosis and cellular proliferation were closely involved in activating MAKP signaling pathway and lipid metabolism pathway in both treated groups. Collectively, these data demonstrate that head kidney of juvenile common carp mainly leverages upregulation of genes related to cell proliferation and turnover, apoptosis, and lipid metabolism to combat sub-chronic exposure of GLY. This study casts new understanding into the risk of GLY in aquatic animals. Graphical abstract: Image 1 Highlights: GLY exposure (5 and 50 mg/L for 45 d) increases the generation of ROS and MDA. Transcriptome analysis identifies 1381 DEGs between the control and treated groups. GLY-induced tp53 may play a vital role in the linkage of a cascade of signals. Head kidney mainly leverages upregulation of related genes to combat GLY.