Multivalent Polyanionic 2D Nanosheets Functionalized Nanofibrous Stem Cell‐based Neural Scaffolds. (10th March 2021)

• Record Type: Journal Article
• Authors: Xia, Yi; Yang, Hua; Li, Shuang; Zhou, Suqiong; Wang, Liyun; Tang, Yuanjiao; Cheng, Chong; Haag, Rainer
• Is Part Of: Advanced functional materials. Volume 31:Number 20(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-03-10
• Subjects: bio‐adhesive graphene nanostructures -- multivalent polyanions -- nanofibrous scaffolds -- neural differentiation -- stem cell regeneration; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202010145
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Because developed neural cells are no longer regenerative and proliferative, achieving neural regenerations by using induced pluripotent stem cells (IPS cells) for nerve diseases have recently attracted much attention. Since the IPS cells' growth and differentiation can be manipulated by different physical and chemicals cues, scaffolds combining the beneficial nanostructures and extracellular matrix may become an ideal interface to promote IPS cells' neural differentiation. In this work, a biocompatible and multivalent polyanion, hyperbranched polyglycerol sulfate, is used to modify the graphene oxide to obtain bio‐adhesive 2D nanosheets. After coating electrospinning nanofibers, the 2D nanosheets‐functionalized nanofibrous scaffolds are applied to mediate the proliferation, lineage specification, and neural differentiation of IPS cells. The results suggest that the modified scaffolds can improve the adhesion and proliferation of IPS cells combined with high efficiency in maintaining their stemness. During the neural differentiation process, the scaffolds can promote neural differentiation and their maturity, meanwhile decreasing the lineage specification toward astrocyte. Overall, this study not only provides new multivalent/bio‐adhesive nanofibrous scaffolds that integrate the chemical and physical cues to facilitate the targeted neural differentiation of IPS cells but also presents a novel pathway for the fabrication of carbon nanomaterials‐based biocomposites in regenerative therapies. Abstract : A biocompatible, bio‐adhesive, and multivalent polyanionic 2D nanosheet is synthesized to fabricate nanofibrous stem cells‐based neural scaffolds. The engineered scaffolds can not only improve the adhesion and proliferation of induced pluripotent stem cells but also augment the neural differentiation and maturity with decreased lineage specification toward astrocyte, which provides a new pathway for fabricating nanofibrous scaffolds in neural regeneration.