Real-time detection and imaging of exogenous and endogenous Zn2+ in the PC12 cell model of depression with a NIR fluorescent probe. Issue 12 (17th May 2021)

• Record Type: Journal Article
• Authors: Feng, Jing; Li, Ji-Zhen; Mao, Xi-Mo; Wang, Qi; Li, Su-Ping; Wang, Cheng-Yun
• Is Part Of: Analyst. Volume 146:Issue 12(2021)
• Page Start: 3971
• Page End: 3976
• Publication Date: 2021-05-17
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an00508a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Depression is closely related to overactivation of N -methyl-D -aspartic acid (NMDA) receptors, and Zn 2+ is a vital NMDA receptor modulator involved in the pathophysiological and physiological processes of depression. Abstract : Depression is closely related to overactivation of N -methyl-d -aspartic acid (NMDA) receptors, and Zn 2+ is a vital NMDA receptor modulator involved in the pathophysiological and physiological processes of depression. Therefore, quantitative and real-time detection of Zn 2+ is very important for understanding the pathogenesis of depression. In this work, a near-infrared (NIR) fluorescent probe ISO-DPA was designed and synthesized for Zn 2+ detection with a large Stokes shift (185 nm), high quantum yield (up to 44%), high sensitivity (LOD = 0.106 μM) and good pH stability. The probe showed rapid response within 10 s, accompanied by a distinct fluorescence change from faint to bright pink with the fluorescence intensity increasing 4.5-fold. Moreover, the sensing mechanism of ISO-DPA towards Zn 2+ was supported by MALDI-TOF-MS and Job's plot. The probe ISO-DPA could detect instantaneous variation of exogenous and endogenous Zn 2+ in PC12 cells. The bioimaging results reveal the increase of the endogenous Zn 2+ concentration in PC12 cells under the oxidative stress induced by glutamate and confirm that overactivation of NMDA receptors results in an increase of the Zn 2+ level. All the results proved that ISO-DPA is an excellent probe for detecting Zn 2+ in solution and living cells and could help us better understand Zn 2+ associated pathogenesis of depression.