Retracted: miR‐145 eliminates lipopolysaccharides‐induced inflammatory injury in human fibroblast‐like synoviocyte MH7A cells. Issue 12 (7th September 2018)

• Record Type: Journal Article
• Authors: Zhong, Feng; Xu, Jian; Yang, Xirui; Zhang, Qi; Gao, Zhaomeng; Deng, Yao; Zhang, Lei; Yu, Chunyan
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 12(2018)
• Page Start: 10059
• Page End: 10066
• Publication Date: 2018-09-07
• Subjects: inflammation -- lipopolysaccharides (LPS) -- MH7A cell -- miR‐145 -- rheumatoid arthritis (RA); Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.27341
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Recently, it has been accepted that miR‐based therapy may be beneficial for rheumatoid arthritis (RA). This study aimed to evaluate the potential involvement of miR‐145 in RA in vitro. The expression of miR‐145 in the human fibroblast‐like synoviocyte line MH7A was overexpressed by miR‐mimic transfection, after which cells were subjected to lipopolysaccharides (LPS). Cell viability, apoptosis, and the release of pro‐inflammatory cytokines were measured. The result showed that the apoptosis and the release of IL‐1β, IL‐6, IL‐8, and TNF‐α were significantly induced by LPS. Meanwhile, LPS treatment led to downregulation of miR‐145. miR‐145 overexpression in LPS‐untreated MH7A cells had no impacts on cell apoptosis and inflammation. But, restoring miR‐145 expression in LPS‐stimulated cells by supplementation of a miR‐145 mimic protected MH7A cells against LPS‐induced apoptosis and inflammation. Furthermore, miR‐145 overexpression in LPS‐untreated MH7A cells slightly blocked the PI3K/ATK and mTOR pathways, whereas miR‐145 overexpression in LPS‐stimulated cells notably repressed the LPS‐induced activation of PI3K/ATK and MAPK/mTOR pathways. Our study suggested that miR‐145 protected MH7A cells against LPS‐induced apoptosis and inflammation by inhibiting the PI3K/AKT and MAPK/mTOR pathways. Abstract : Recently, it has been clear that miR‐based therapy may be beneficial for rheumatoid arthritis (RA). This study aimed to evaluate the beneficial potential of miR‐145 in RA in vitro. Our study suggested that miR‐145 protected MH7A cells against LPS‐induced apoptosis and inflammation by inhibiting PI3K/AKT and MAPK/mTOR pathways.