The Homologous Regions of Spodoptera litura (Lepidoptera: Noctuidae) Nucleopolyhedrovirus II Have Both the Function as Origin of DNA Replication and Enhancer. Issue 1 (2nd July 2015)

• Record Type: Journal Article
• Authors: Liu, Huifen; Gao, Huiju; Guo, Guang; Li, Yunzhi; Li, Yinü; Wang, Jinhui; Zhang, Zhifang; Yu, Zhencheng
• Is Part Of: Journal of insect science. Volume 15:Issue 1(2015)
• Publication Date: 2015-07-02
• Subjects: homologous region (hr) -- DNA replication origin -- enhancer -- nucleopolyhedrovirus -- Spodoptera litura; Insects -- Periodicals; Entomology -- Periodicals; Arthropoda -- Periodicals; Insects; Arthropods; Biology; Ecology; Entomologie -- Périodiques; Entomology; Insects; Electronic journals; Periodicals; 595.05
• DOI: 10.1093/jisesa/iev068
• Languages: English
• ISSNs: 1536-2442

Abstract

Abstract : In the genome sequence of the Spodoptera litura nucleopolyhedrovirus II (SpltNPVII), seven homologous regions ( hr s), Sp hr 1-7, were identified. Each of them composed of three to eight 64-bp highly conserved sequences, and each contained a 24-bp imperfect palindrome. A transient expression assay demonstrated that the expression of SpltNPVII -ie1 promoter-driven luciferase gene was enhanced between 3- and 13-fold by infection of SpltNPVII in Spli221 cells. Real-time polymerase chain reaction confirmed each of seven hr s could function as origin ( ori ) of viral DNA replication. This suggests that these hr s are bifunctional, having both ori and enhancer activities for transcription. In addition, the potential of seven hr s as origins had a significantly positive correlation with the number of their palindromes ( r = 0.847, Sig: 0.016 < 0.05), and enhancer efficiency had a significantly positive correlation with the number of characteristic motifs ( r = 0.893, Sig: 0.007 < 0.01). The efficiency of replication and enhancement of each hr both increased with increasing total numbers of palindromes, repeat sequences, and characteristic motifs. In addition, a single 64-bp highly conserved consensus sequence cannot very good support to the function as origin and enhancer, and require the assistance of other cis -elements in hr s.