Peptidoglycan Recognition Protein S2 From Silkworm Integument: Characterization, Microbe-Induced Expression, and Involvement in the Immune-Deficiency Pathway. Issue 1 (22nd March 2015)

• Record Type: Journal Article
• Authors: Yang, Jie; Wang, Xiaonan; Tang, Shunming; Shen, Zhongyuan; Wu, Jinmei
• Is Part Of: Journal of insect science. Volume 15:Issue 1(2015)
• Publication Date: 2015-03-22
• Subjects: Bombyx mori -- innate immunity -- peptidoglycan recognition protein -- RNA interference; Insects -- Periodicals; Entomology -- Periodicals; Arthropoda -- Periodicals; Insects; Arthropods; Biology; Ecology; Entomologie -- Périodiques; Entomology; Insects; Electronic journals; Periodicals; 595.05
• DOI: 10.1093/jisesa/iev007
• Languages: English
• ISSNs: 1536-2442

Abstract

Abstract : Peptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori . A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP ( DmPGRP-SA ). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli - and Bacillus subtilis- challenged B. mori was measured using semiquantitative reverse transcriptase polymerase chain reaction analysis. The expression of BmPGRP-S2 was upregulated at 24 h by E. coli and Ba. subtilis challenge. In addition, in the integument of B. mori, RNAi knockdown of BmPGRP-S2 caused an obvious reduction in the transcription expression of the transcription factor Relish and in antibacterial effector genes Attacin, Gloverin, and Moricin . The results indicated that BmPGRP-S2 participates in the signal transduction pathway of B. mori .