Histidine polypeptide-hybridized nanoscale metal–organic framework to sense drug loading/release. (July 2021)

• Record Type: Journal Article
• Authors: Xu, Yanan; Li, Zhenhua; Xiu, Dan; Sun, Guotao; Snow, Christopher D.; Wang, Yao; Wang, Yanxin; Belfiore, Laurence A.; Tang, Jianguo
• Is Part Of: Materials & design. Volume 205(2021)
• Publication Date: 2021-07
• Subjects: ZIF-8 -- 5-FAM -- Histidine polypeptide -- Drug loading and release -- Fluorescence response; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2021.109741
• Languages: English
• ISSNs: 0264-1275

Abstract

Graphical abstract: Highlights: MOFs was synthesized through one-pot synthesis at room temperature. Real-time observation of doxorubicin loading/release from MOFs by fluorescence. Fluorescence intensity enhancement after the pH-triggered doxorubicin release. The addition of histidine polypeptides increases uptake by HepG-2 cells. Abstract: Histidine Polypeptide-Hybridized Nanoscale Metal–Organic Framework (fH12/ZIF-8) can be produced effectively in water at room temperature without using any additives. The fluorescein label also provides a useful method to quantify the extent to which the anti-cancer drug doxorubicin (DOX) is loaded into or released from the resulting MOFs. In vitro studies have shown that fH12/ZIF-8 has the useful property of dissolving and controlled rates at low pH. It ruptured fastest in PBS buffer solution with pH 6.3, and the release rate reached 50% at 4 h. Furthermore, it showed that the addition of histidine polypeptides increases uptake by HepG-2 cells. This study integrates drug loading and detecting aspects into a single theranostic platform by a simple and efficient strategy, opening a new route for the construction of advanced drug delivery systems.